Our experienced research team, lead by Dr. Jack Vanden Heuvel (see link), will work with you in your research on nuclear receptors. We can help create high-throughput NR reporter gene assays, develop ortholog assays or a variety of other services. Please contact an INDIGO Biosciences representative for more details at (814) 234-1919, customerserv@indigobiosciences.com or click on the Request Info page.

Background: INDIGO Biosciences wants to help you perform research on nuclear receptors and evaluate compounds of interest. We have an experienced research team that can provide expertise in nuclear receptor biology and their role in drug and nutrient activity and toxicity. 

Examples: 

Custom Nuclear Receptor Assay. Interested in a human nuclear receptor that is not in our current portfolio? We can clone and optimize an assay for use here in our laboratories.

Ortholog Nuclear Receptor Assay. Many labs are interested in cross-species comparisons, especially contrasting human NR activity to common laboratory animals such as rat, mouse, dog and non-human primate. Although we offer many NR assays in human, rat and mouse, if you need an assay for other species, our services lab can clone and optimize an assay. 

Selective Receptor Modulation (SRM) Assays.  The concept that ligands for members of the NR superfamily should be classified either as ‘agonists’ or as ‘antagonists’ is often an oversimplification. For example, tamoxifen acts in vivo as an estrogen receptor (ER) antagonist in the breast but as an ER agonistic in bone and uterus. The reason for these opposite actions is provided by numerous NR-associated proteins such as corepressors/coactivators that interact with a particular ligand receptor complex. Thus a new concept of selective receptor modulators (SRMs) has emerged to account for the different contextual actions of NR ligands, depending on the cellular milieu in which this chemical acts. INDIGO has at its disposal a variety of coactivator and corepressor constructs, as well as LXXLL containing peptides for examination of protein-protein interaction in the mammalian two-hybrid assay.

Nuclear Receptor Assay in a Specific Cell Line. INDIGO services has optimized its reporter assays in particular cell lines (may vary depending on the receptor). If this cell lines not where you need the information, let INDIGO's staff know and we can develop and optimize the appropriate conditions.

Nuclear Receptor Assay with a Specific Reporter. Similar to using a specific cell line, if our optimized reporter gene is not what you need, INDIGO will incorporate a different reporter gene in the customized assay. 

Deliverable: Custom assays are developed under a non-exclusive arrangement. Although the constructs developed for the client may be sent for their own use, INDIGO retains the right to add the newly developed assay as part of the services portfolio. 

Price: Please inquire. 

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