Human RXRα (NR2B1)
Family of Reporter Assay Systems

INDIGO's RXR Alpha Reporter Assay System utilizes non-human mammalian cells engineered to provide constitutive, high-level expression of Human Retinoid X Receptor Alpha (NR2B1), a ligand-dependent transcription factor commonly referred to as RXRA or RXRα. Additionally, these cells contain an RXRα-responsive luciferase reporter gene. Thus, quantifying luciferase activity provides a surrogate measure of RXRα activity in the treated reporter cells.

Kits are offered in different assay formats to accommodate researchers' needs: 3x 32, 1x 96, and 1x 384 assay formats for screening small numbers of test compounds, as well as custom bulk reagents for HTS applications. Assay systems are all inclusive, providing reporter cells, optimized growth media, media for diluting test compounds, a positive-control agonist, luciferase detection reagent, a white assay plate, a detailed protocol, and a protocol quick guide. All kits are shipped on dry ice.