We offer this powerful technique to quantitate changes in the expression of mRNA for genes of interest. This service allows accurate monitoring of the effects of your investigational compounds on the transcription of particular genes. This technique is a good follow-up to validate results of microarray studies.
Control real time PCR analysis for a standard dilution of actin.
References:
Tien, E.S., Gray, J.P., Peters, J.M., and Vanden Heuvel, J.P. “Comprehensive Gene Expression Analysis of Peroxisome Proliferator-Treated Immortalized Hepatocytes: Identification of Peroxisome Proliferator-Activated Receptor alpha-dependent Growth Regulatory Genes” Cancer Res., 2003, 63, 5767-5780.
Tien, E.S., Davis, J.W., and Vanden Heuvel, J.P. “Identification of the CREB-binding Protein/p300-interacting Protein CITED2 as a Peroxisome Proliferator-activated Receptor alpha Coregulator” J. Biol. Chem., 2004, 279, 24053-24063.
