Mouse Peroxisome Proliferator-Activated Receptor Alpha (mPPARα; Nr1c1)
Family of Reporter Assay Systems
INDIGO's Mouse Peroxisome Proliferator-Activated Receptor Alpha (mPPARα) Reporter Assay System utilizes proprietary mammalian cells engineered to provide constitutive, high-level expression of Mouse Ppara (Nr1c1), a ligand-dependent transcription factor. Because these cells incorporate a responsive luciferase reporter gene, quantifying expressed luciferase activity provides a sensitive surrogate measure of mPPARα activity in treated cells. The primary application of this reporter assay system is in the screening of test samples to quantify functional activity, either agonist or antagonist, that they may exert against mouse PPARα.
Mouse PPARα Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.
|Product Description||Product Number||Technical Manual||Protocol Quick Guide|
|Mouse PPARα Reporter Assay System 3x 32 assays in 96-well format||M00111-32|
|Mouse PPARα Reporter Assay System 1x 96-well format assay||M00111|
|Mouse PPARα Reporter Assay System 1x 384-well format assays||M00112|