Human Androgen Receptor (AR; NR3C4)
Family of Reporter Assay Systems
This nuclear receptor assay system utilizes proprietary non-human mammalian cells engineered to provide constitutive, high-level expression of full length, unmodified Human Androgen Receptor (NR3C4), a ligand-dependent transcription factor commonly referred to as AR. INDIGO's Reporter Cells include the luciferase reporter gene functionally linked to a bona fide AR-responsive promoter. Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in AR activity. Luciferase gene expression occurs after ligand-bound AR undergoes nuclear translocation, DNA binding, recruitment and assembly of the co-activators and accessory factors required to form a functional transcription complex, culminating in expression of the target gene. Unlike some other cell-based assay strategies, the readout from INDIGO's reporter cells demands the same orchestration of all intracellular molecular interactions and events that can be expected to occur in vivo.
AR Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.
The principle application of this assay product is in the screening of test samples to quantify functional activities, either agonist or antagonist, that they may exert against the human androgen receptor. This kit product is an all-inclusive assay system that includes, in addition to AR Reporter Cells, two optimized media for use during cell culture and (optionally) in diluting the test samples, a reference agonist, Luciferase Detection Reagent, a cell culture-ready assay plate, and a detailed protocol.
|Product Description||Product Number||Technical Manual||Protocol Quick Guide|
|Human AR Reporter Assay System 3x 32 assays in 96-well format||IB03001-32|
|Human AR Reporter Assay System 1x 96-well format assay||IB03001|
|Human AR Reporter Assay System (Bulk Pack) Bulk Reagent Pack for 10x 96-well plates||IB03001-B10|
|Human AR Reporter Assay System 1x 384-well format assays||IB03002|
|Human AR Reporter Assay System (Bulk Pack) Bulk Reagent Pack for 10x 384-well plates||IB03002-B10|
|Alternative volumes of Bulk Assay Reagents may be custom manufactured to better accommodate Customer needs. Please Inquire.|