We offer several methods to study adipogenesis.
PPARγ plays important roles in control of adipogenesis. We offer PPARγ reporter assays as a useful tool for studies of this process.
We offer an Oil Red O Assay on 3T3-L1 preadipocytes as an in vitro model system for lipid accumulation.
The fatty acid binding protein aP2 is an adipocyte marker. We offer an aP2 reporter assay for studies of this protein.
Oil Red O adipocyte differential assay in 3T3-L1 cells. DMSO represents vehicle control. Abbreviations: CLA, conjugated linoleic acid; ABT, 1-aminobenzotriazole; IBU, ibuprofen; NDGA, nordihydroguaiaretic acid; BADGE, bisphenol A diglycidyl ether.
References:
Khan, S.A. and Vanden Heuvel, J.P. “Role of Nuclear Receptors in the Regulation of Gene Expression by Dietary Fatty Acids” J. Nutr. Biochem., 2003, 14, 554-567.
